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Tools
BLAST
Protein Query
Protein to Nucleotide BLAST (tblastn)
Protein to Nucleotide BLAST (tblastn)
Request a New BLAST
Enter
Protein
Query Sequence
Enter one or more queries in the top text box or use the browse button to upload a file from your local disk. The file may contain a single sequence or a list of sequences. In both cases, the data must be in
FASTA format
.
Show an Example Sequence
Enter FASTA sequence(s)
Or upload your own query FASTA:
Upload
The file should be a plain-text FASTA (.fasta, .fna, .fa, .fas) file. In other words, it cannot have formatting as is the case with MS Word (.doc, .docx) or Rich Text Format (.rtf). It cannot be greater than
2MB
in size.
Don't forget to press the Upload button before attempting to submit your BLAST.
Choose Search Target
Choose from one of the
nucleotide
BLAST databases listed below. You can also use the browse button to upload a file from your local disk. The file may contain a single sequence or a list of sequences.
Nucleotide
BLAST Databases:
Select a Dataset
ASFV Public Strain Database (updated 09-Mar-2021)
CSFV Public Strain Database (updated 09-Mar-2021)
FMDV Public Strain Database (updated 09-Mar-2021)
PDCoV Public Strain Database (updated 09-Mar-2021)
PEDV Public Strain Database (updated 09-Mar-2021)
PRRSV Public Strain Database (updated 09-Mar-2021)
SVA Public Strain Database (updated 09-Mar-2021)
US-SPD Aggregated Public Strain Database (updated 09-Mar-2021)
Or upload your own dataset:
Upload
The file should be a plain-text FASTA (.fasta, .fna, .fa) file. In other words, it cannot have formatting as is the case with MS Word (.doc, .docx) or Rich Text Format (.rtf). It cannot be greater than
2MB
in size.
Don't forget to press the Upload button before attempting to submit your BLAST.
Advanced Options
General parameters
Max target sequences:
10
50
100
250
500
e-Value (Expected Threshold)
Expected number of chance matches in a random model. This number should be give in a decimal format.
More Information
|
Expect value vedio tutorial
Word size:
3
6
Scoring parameters
Matrix
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Gap Costs:
Existence: 11 Extension: 2
Existence: 10 Extension: 2
Existence: 9 Extension: 2
Existence: 8 Extension: 2
Existence: 7 Extension: 2
Existence: 6 Extension: 2
Existence: 13 Extension: 1
Existence: 12 Extension: 1
Existence: 11 Extension: 1
Existence: 10 Extension: 1
Existence: 9 Extension: 1
BLAST